Molecular Weight:Article Title: M13 phage based gene therapy platform
Article Snippet: .. Phage Protein Function # of Amino Acids Molecular Weight p2 DNA replication 410 46,137 p10 DNA replication 111 12,672 p5 binding of ssDNA 87 9,682 p8 major coat capsid 50 5,235 p3 minor tail capsid 406 42,522 p6 minor tail capsid 112 12,342 p7 minor head capsid 33 3,599 p9 minor head capsid 32 3,650 p1 assembly 348 39,502 p4 assembly 405 43,502 p11 assembly 108 12,424 Phage Quantification Phage concentrations are given by the absorbance peak at 269 nm with correction at 320 nm (read by NanoDrop) and the number of nucleotides in the given phage genome: Phage Concentration = ( A 269 - A 320 ) ( 6 × 10 17 ) number of nts of phage genome particles / ml Givens: 330 daltons per base and molar extinction coefficient of 1.006×104 M−1cm−1104 .. C1 Cloning of Vector Variants Insertion or Restriction Sites into the RM13-f1 Plasmid Restriction sites were inserted into the p3 (KpnT/EagI) and p8 (PstI/BamHI) DNA sequences using two-fragment Gibson assembly reactions.
Binding Assay:Article Title: M13 phage based gene therapy platform
Article Snippet: .. Phage Protein Function # of Amino Acids Molecular Weight p2 DNA replication 410 46,137 p10 DNA replication 111 12,672 p5 binding of ssDNA 87 9,682 p8 major coat capsid 50 5,235 p3 minor tail capsid 406 42,522 p6 minor tail capsid 112 12,342 p7 minor head capsid 33 3,599 p9 minor head capsid 32 3,650 p1 assembly 348 39,502 p4 assembly 405 43,502 p11 assembly 108 12,424 Phage Quantification Phage concentrations are given by the absorbance peak at 269 nm with correction at 320 nm (read by NanoDrop) and the number of nucleotides in the given phage genome: Phage Concentration = ( A 269 - A 320 ) ( 6 × 10 17 ) number of nts of phage genome particles / ml Givens: 330 daltons per base and molar extinction coefficient of 1.006×104 M−1cm−1104 .. C1 Cloning of Vector Variants Insertion or Restriction Sites into the RM13-f1 Plasmid Restriction sites were inserted into the p3 (KpnT/EagI) and p8 (PstI/BamHI) DNA sequences using two-fragment Gibson assembly reactions.
Concentration Assay:Article Title: M13 phage based gene therapy platform
Article Snippet: .. Phage Protein Function # of Amino Acids Molecular Weight p2 DNA replication 410 46,137 p10 DNA replication 111 12,672 p5 binding of ssDNA 87 9,682 p8 major coat capsid 50 5,235 p3 minor tail capsid 406 42,522 p6 minor tail capsid 112 12,342 p7 minor head capsid 33 3,599 p9 minor head capsid 32 3,650 p1 assembly 348 39,502 p4 assembly 405 43,502 p11 assembly 108 12,424 Phage Quantification Phage concentrations are given by the absorbance peak at 269 nm with correction at 320 nm (read by NanoDrop) and the number of nucleotides in the given phage genome: Phage Concentration = ( A 269 - A 320 ) ( 6 × 10 17 ) number of nts of phage genome particles / ml Givens: 330 daltons per base and molar extinction coefficient of 1.006×104 M−1cm−1104 .. C1 Cloning of Vector Variants Insertion or Restriction Sites into the RM13-f1 Plasmid Restriction sites were inserted into the p3 (KpnT/EagI) and p8 (PstI/BamHI) DNA sequences using two-fragment Gibson assembly reactions.
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